Protoplast Formation and Regeneration in Acetobacter Pasteurianus
نویسندگان
چکیده
Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as an osmotic stabilizer and lysozyme (0.5mg/ml). Addition of mutanolysin did not show enhancement in protoplast formation. Maximum protoplast regeneration was obtained on Acetobacter medium with NaCl (0.5M) as an osmotic stabilizer. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of Acetobacter pasteurianus.
منابع مشابه
Complete Genome Sequences of Two Acetic Acid-Producing Acetobacter pasteurianus Strains (Subsp. ascendens LMG 1590T and Subsp. paradoxus LMG 1591T)
Citation: Jia B, Chun BH, Cho GY, Kim KH, Moon JY, Yeo S-H and Jeon CO (2017) Complete Genome Sequences of Two Acetic AcidProducing Acetobacter pasteurianus Strains (Subsp. ascendens LMG 1590 and Subsp. paradoxus LMG 1591T). Front. Bioeng. Biotechnol. 5:33. doi: 10.3389/fbioe.2017.00033 Complete Genome Sequences of two acetic acid-producing Acetobacter pasteurianus Strains (Subsp. ascendens LMG...
متن کاملAcetic acid fermentation of acetobacter pasteurianus: relationship between acetic acid resistance and pellicle polysaccharide formation.
Acetobacter pasteurianus strains IFO3283, SKU1108, and MSU10 were grown under acetic acid fermentation conditions, and their growth behavior was examined together with their capacity for acetic acid resistance and pellicle formation. In the fermentation process, the cells became aggregated and covered by amorphous materials in the late-log and stationary phases, but dispersed again in the secon...
متن کاملA novel high ethanol-thermo-tolerant Acetobacter pasteurianus KBMNS-IAUF-2 strain and the optimization of acetic acid production using the Taguchi statistical method
Because of the high energy consumption for fermentor cooling, the isolation of thermo-tolerant Acetobacter strains for vinegar production has a high priority. The aims of this study were the isolation and identification of a high ethanol-thermo-tolerant Acetobacter spp. from grapes as well as the optimization of conditions for increasing the acetic acid production. The grape e...
متن کاملA novel high ethanol-thermo-tolerant Acetobacter pasteurianus KBMNS-IAUF-2 strain and the optimization of acetic acid production using the Taguchi statistical method
Because of the high energy consumption for fermentor cooling, the isolation of thermo-tolerant Acetobacter strains for vinegar production has a high priority. The aims of this study were the isolation and identification of a high ethanol-thermo-tolerant Acetobacter spp. from grapes as well as the optimization of conditions for increasing the acetic acid production. The grape e...
متن کاملComplete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, Acetobacter pasteurianus SKU1108, Provide a New Insight into Thermotolerance
Acetobacter pasteurianus SKU1108 is a typical thermotolerant acetic acid bacterium. In this study, the complete genome sequence of the SKU1108 strain was elucidated, and information on genomic modifications due to the thermal adaptation of SKU1108 was updated. In order to obtain a clearer understanding of the genetic background responsible for thermotolerance, the SKU1108 genome was compared wi...
متن کامل